Neuropharmacology Poster Session



Materials & Methods


Discussion & Conclusion



INABIS '98 Home Page Your Session Symposia & Poster Sessions Plenary Sessions Exhibitors' Foyer Personal Itinerary New Search

Characterization of Quinpirole-Stimulated [35S] GTPgammaS Binding at D2-like Receptors in Rat Striatum.

Contact Person: Sarah L. Gilliland (sgillila@kumc.edu)


Quinpirole (LY171555) is a selective D2-like receptor agonist used extensively in vivo and in vitro. [3H]Quinpirole binds selectively to D2-like receptors in vitro (Levant et al., JPET, 262 (3) 929-935, 1992). Surprisingly, the binding of [3H]quinpirole to D2-like sites in rat striatum is competitively inhibited by the monoamine oxidase inhibitors (MAOIs) Ro 41-1049, clorgyline, (+)-deprenyl and (-)-deprenyl (Levant et al., EJP, 241:141, 1993). In addition, the MAOI clorgyline has been shown to block the development and maintenance of quinpirole-induced behavioral sensitization (Culver and Szechtman, EJP, 339:109-111, 1997) suggesting that MAOI interactions at quinpirole labeled sites have functional importance.

The function of G protein-coupled receptors, which include D2-like receptors, can be assessed by agonist-stimulated [35S]GTPgammaS binding in crude membrane preparations. This provides a measure of G protein activation, the initial step in signal transduction.

The PURPOSES of these studies were to: 1) characterize quinpirole-stimulated [35S]GTPgammaS binding in rat striatal membranes, and 2) determine if MAOIs modulate quinpirole-stimulated [35S]GTPgammaS binding.

Back to the top.

<= Abstract INTRODUCTION Materials & Methods =>

| Discussion Board | Next Page | Your Poster Session |
Gilliland, S.; Alper, R.; Levant, B.; (1998). Characterization of Quinpirole-Stimulated [35S] GTPgammaS Binding at D2-like Receptors in Rat Striatum.. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/neuropharm/gilliland0401/index.html
© 1998 Author(s) Hold Copyright