Neuropharmacology Poster Session
Materials and Methods
Quinpirole-stimulated [35S]GTPgammaS binding was assessed in striatal membranes prepared from adult male Sprague Dawley rats. Each assay tube contained agonist and/or test drug(s), 300 microM GDP, 0.1 nM [35S]GTPgammaS and membrane homogenate (40 - 60 micrograms protein), in a final buffer concentration of 20mM Hepes, 100mM NaCl, and 3mM MgCl2, pH 7.4. Reaction tubes were incubated at 37o C for 20 minutes, then terminated by rapid vacuum filtration. [35S]GTPgammaS binding was quantified by scintillation spectroscopy. Data are presented as percent stimulation over basal. The EC50, IC50, and Emax values were calculated by nonlinear regression analysis using a four parameter model (Sigma Plot, SPSS, Chicago, IL).
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|Gilliland, S.; Alper, R.; Levant, B.; (1998). Characterization of Quinpirole-Stimulated [35S] GTPgammaS Binding at D2-like Receptors in Rat Striatum.. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/neuropharm/gilliland0401/index.html|
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