Invited Symposium: Oxidative Stress and the CNS
Materials and Methods
For studies of SAA deficiency, female Long-Evans adult rats were provided for 5 days with either a sulfur-deficient diet (38, 39) or a control diet supplemented with L-cystine and L-methionine. For this and the study below, tissues were perfused prior to collection to eliminate blood contamination. GSH concentration was analyzed in neocortex, hippocampus, thalamus, striatum, and liver by reverse-phase high performance liquid chromatography using ultraviolet detection and precolumn derivatization with 5,5'-dithio-bis(2-nitrobenzoic acid); the method was modified from that of Komura et al. (40).
To investigate the impact of Se status, weanling, male Long-Evans rats were fed for 6 weeks diets (38, 39) containing deficient, control, or supplemental levels of Se. Total GPX was measured in liver, thalamus, and neocortex by a modification of the method of Prohaska and Ganther (37).
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|Paterson, P.G.; (1998). Nutritional Regulation of Peroxide Scavenging. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/juurlink/paterson0526/index.html|
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