Cell Biology Poster Session



Materials & Methods


Discussion & Conclusion



INABIS '98 Home Page Your Session Symposia & Poster Sessions Plenary Sessions Exhibitors' Foyer Personal Itinerary New Search

Balance Between Activated-STAT And MAP Kinase Regulates The Growth of Human Bladder Cell Lines After Treatment With EGF

Contact Person: Hitoshi Kawamata (kawamata@dent.tokushima-u.ac.jp)


  1. Berger, M. S., Greenfield, C., Gullick, W. J., Downward, H. J., Neal, D. E., Harris, A. L., and Waterfield, M. D., Evaluation of epidermal growth factor receptors in bladder tumors. Br. J. Cancer, 56, 533-537 (1987).
  2. Bubenik, J., Baresora, M, Viklicky, V., Jakoubkova, J., Sainerova, H., and Donner, J., Established cell line of urinary bladder carcinoma (T-24) containing tumor specific antigen. Int. J. Cancer, 11, 765-773 (1973).
  3. Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D., and Mitchell, J. B., Evaluation of a tetrazolium-based semiautomated calorimetric assay: assessment of chemosensitivity testing. Cancer Res., 47, 936-942 (1987).
  4. Chin, Y. E., Kitagawa, M., Su, W-C. S., You, Z-H., Iwamoto, Y., and Fu, X-Y., Cell growth arrest and induction of cyclin-dependent kinase inhibitor p21waf1/cip1 mediated by STAT1. Science, 272, 719-722 (1996).
  5. Cooper, M. J., Haluschak, J. J., Johnson, D., Schwartz, S., Morrison, L. J., Lippa, M., Hatzivassiliou, G., and Tan, J., p53 mutations in bladder carcinoma cell lines. Oncol. Res., 6, 569-579 (1994).
  6. David, M., Wong, L., Flavell, R., Thompson, S. A., Wells, A., Larner, A. C., and Johnson, G. R., STAT activation by epidermal growth factor (EGF) and amphiregulin. Requirement for the EGF receptor kinase but not for tyrosine phosphorylation sites or JAK1. J. Biol. Chem., 271, 9185-9188 (1996).
  7. Darnell Jr., J. E., STATs and gene regulation. Science, 277, 1630-1635 (1997).
  8. El-Deiry, W. S., Tokino, T., Velculescu, V. E., Levy, D. B., Parsons, R., Trent, J. M., Lin, D., Mercer, W. E., Kinzler, K. W., and Vogelstein, B., WAF1, a potential mediator of p53 tumor suppression. Cell, 75, 817-825 (1993).
  9. Fan, Z., Lu, Y., Wu, X., DeBlasio, A., Koff, A., and Mendelsohn, J., Prolonged induction of p21Cip1/WAF1/CDK2/PCNA complex by epidermal growth factor receptor activation mediates ligand-induced A431 cell growth inhibition. J. Cell. Biol., 131, 235-242 (1995).
  10. Fujimoto, K., Tanaka, Y., Rademaker, A., and Oyasu, R., Epidermal growth factor-responsive and refractory carcinomas initiated with N-methyl-N-nitrosourea in rat urinary bladder. Cancer Res., 56, 2666-2670 (1996).
  11. Jakus, J., and Yeudall, W. A., Growth inhibitory concentrations of EGF induce p21 (WAF1/Cip1) and alter cell cycle control in squamous carcinoma cells. Oncogene, 12, 2369-2376 (1996).
  12. Kameyama, S., Kawamata, H., Pastan, I., and Oyasu, R., Cytotoxic effect of a fusion protein combining transforming growth factor a with pseudomonas exotoxin on rat and human bladder carcinoma cells in vitro. J. Cancer Res. Clin. Oncol., 120, 507-512 (1994).
  13. Kawai, K., Kawamata, H., Kameyama, S., rademaker, A., and Oyasu, R., Intraluminal epidermal growth factor affects growth of N-methyl-N-nitrosourea-initiated rat bladder carcinoma. Jpn. J. Cancer Res., 86, 429-434 (1995).
  14. Kawamata, H., Azuma, M., Kameyama, S., Nan, L., and Oyasu, R., Effect of epidermal growth factor/transforming growth factor a and transforming growth factor b1 on growth in vitro of rat urinary bladder carcinoma cells. Cell Growth & Differ., 3, 819-825 (1992).
  15. Kawamata, H., Kameyama, S., and Oyasu, R., In vitro and in vivo acceleration of the neoplastic phenotype of a low-tumorigenicity rat bladder carcinoma cell line by transfected transforming growth factor-a. Mol. Carcinog., 9, 210-219 (1994).
  16. Kawamata, H., Kameyama, S., Nan, L., Kawai, K., and Oyasu, R., Effect of EGF and TGF-b1 on growth and invasive potentials of newly established rat bladder carcinoma cell lines. Int. J. Cancer, 55, 968-973 (1993).
  17. Kawasaki, T., Tomita, Y., Bilim, V., Takeda, M., Takahashi, K., and Kumanishi, T., Abrogation of apoptosis induced by DNA-damaging agents in human bladder-cancer cell lines with p21/WAF1/CIP1 and/or p53 gene alterations. Int. J. Cancer, 68, 501-505 (1996).
  18. Kwok, T. T., Mok, C. H., and Menton-Brennan, L., Up-regulation of mutant form of p53 by doxorubicin in human squamous carcinoma cells. Cancer Res., 54, 2834-2836 (1994).
  19. Leaman, D. W., Pisharody, S., Flickinger, T. W., Commane, M. A., Schlessinger, J., Kerr, I. M., Levy, D. E., and Stark, G. R., Roles of JAKs in activation of STATs and stimulation of c-fos gene expression by epidermal growth factor. Mol. Cell. Biol., 16, 369-375 (1996).
  20. Marshall, C. J., Specificity of receptor tyrosine kinase signaling: Transient versus sustained extracellular signal-regulated kinase activation. Cell, 80, 179-185 (1995).
  21. Messing, E. M., Hanson, P., Ulrich, P., and Erturk, E. Epidermal growth factor-interactions with normal and malignant urothelium: in vivo and in situ studies. J. Urol., 138, 1329-1335 (1987).
  22. Messing, E. M., Clinical implications of the expression of epidermal growth factor receptors in human transitional cell carcinoma. Cancer Res., 50, 2530-2537 (1990).
  23. Momose, H., Kakinuma, H., Shariff, S. Y., Mitchell, G. B., Rademaker, A., and Oyasu, R., Tumor-promoting effect of urinary epidermal growth factor in rat urinary bladder carcinogenesis. Cancer Res., 51, 5487-5490 (1991).
  24. Neal, D., Marsh, C., Bennett, M. K., Abel, P. D., Hall, R., R., Sainsbury, J. R. C., and Harris, A. L., Epidermal growth factor receptors in human bladder cancer: comparison of invasive and superficial tumors. Lancet, i, 366-368 (1985).
  25. Neal, D. E., Sharples, L., Smith, K., Fennelly, J., Hall, R. R., Sainsbury, J. R. C., and Harris, A. L., The epidermal growth factor receptor and the prognosis of bladder cancer. Cancer, 65, 1619-1625 (1990).
  26. Rigby, C. C., and Franks, L. M. A human tissue culture cell line from a transitional cell tumor of the urinary bladder: growth, chromosome pattern and ultrastructure. Brit. J. Cancer, 24, 746-754 (1970).
  27. Sato, M., Kawamata, H., Harada, K., Nakashiro, K., Ikeda, Y., Gohda, H., Yoshida, H., Nishida, T., Ono, K., Kinoshita, M., and Adachi, M., Induction of cyclin-dependent kinase inhibitor, p21waf1, by treatment with 3, 4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone (vesnarinone) in a human salivary cancer cells with mutant p53 gene. Cancer Lett., 112, 181-189 (1997).
  28. Wen, Z., Zhong, Z., and Darnell, Jr. J. E., Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine phosphorylation. Cell, 82, 241-250 (1995).

Back to the top.

<= Discussion & Conclussions REFERENCES

| Discussion Board | Next Page | Your Poster Session |
Kawamata, H.; Hattori, K.; Uchida, D.; Hino, S.; Omotehara, F.; Sato, M.; Oyasu, R.; (1998). Balance Between Activated-STAT And MAP Kinase Regulates The Growth of Human Bladder Cell Lines After Treatment With EGF. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/cellbio/kawamata0333/index.html
© 1998 Author(s) Hold Copyright