Invited Symposium: Molecular and Cellular Analysis of Dopamine and Serotonin Transporters



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Role of the 5-HT Transporter in the Drug-Induced 'Carrier-Dependent' Release

Contact Person: Tiziana Mennini (tiziana@irfmn.mnegri.it)


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Fig.1: d-Fenfluramine induces a carrier-dependent release of unmetabolized [3H]5-HT, both in absence and presence of pargyline. This experiment was carried out in a calcium-free medium to exclude the calcium-dependent component of dF-induced release (suggested to be exocytotic-like and not carrier-mediated, Gobbi et al., 1993; Crespi et al, 1997).

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Fig.2: RO4-1284 induces a dose-dependent tritium release from [3H]5-HT preloaded synaptosomes

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Fig.3: Although quite rapid, the release induced by RO4-1284 is delayed as compared with the dF-induced release

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Fig.4: In the presence of pargyline, the release induced by RO4-1284 is mainly due to unmetabolized [3H]5-HT, while in the absence of pargyline it was mainly due to [3H]5-HIAA.

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Fig.5: However, either in the presence or absence of pargyline, the RO4-1284 induced release is calcium- and carrier-independent

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Gobbi, M; Dalla Valle, F; Ciapparelli, C; Mennini, T; (1998). Role of the 5-HT Transporter in the Drug-Induced 'Carrier-Dependent' Release. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/simantov/gobbi0727/index.html
© 1998 Author(s) Hold Copyright