Pharmacology & Toxicology Poster Session



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the Role of Divalent Cations in the Mechanisms of EDTA Cytotoxicity

Contact Person: Michael O. Ogundele (mogundel@yahoo.com)


EDTA failed to cause any degree of hemolysis of rabbit red blood cells (RRBC) in the absence of additional calcium and magnesium cations, using GVB2- buffer. Mg2+ caused a dose-dependent enhancement of EDTA cytotoxicity, at all concentrations tested, up till 50mM. Ca2+ caused a dose-dependent increase in EDTA-toxicity up to a maximal level of 5mM. Thereafter it caused a progressive inhibition of the EDTA-induced hemolysis in a dose-dependent manner, gradually approaching that of the buffer level

Figure 1: Effect of calcium and magnesium cations on EDTA toxicity in *GVB++ (using unsensitized rabbit erythrocytes as target cells) * GVB2- was used to assess the toxicity of EDTA without any cations


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Ogundele, M.O.; (1998). the Role of Divalent Cations in the Mechanisms of EDTA Cytotoxicity. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/pharmtox/ogundele0173/index.html
© 1998 Author(s) Hold Copyright