Pharmacology & Toxicology Poster Session
Materials & Methods
Discussion & Conclusion
Calcium Imaging Of Smooth Muscle Cells Cultured From Different Regions OF Human And Guinea Pig Lower Urinary Tract.
Contact Person: Jochen Neuhaus (email@example.com)
Materials and Methods
Samples from bladder dome and bladder neck of human and guinea-pig and from prostate (TUR-P) were cut into small pieces (0.5 mm diameter). Those explants were cultured on plastic dishes (35 mm), which were used pure or coated either with collagen A, laminin or poly-l-lysine in a humidified atmosphere with 5% CO2 at 37C.
To characterize the cell population we did immunocytochemical stainings for various antigens and BrdU pulse labelling to examine cell cycle. For calcium-imaging experiments subconfluent cultures were incubated in 2.5 M fura-2 (Molecular Probes) for 30 min at 37C. Following a wash in oxygened Krebs-Ringer cells were stimulated with different neurotransmitters and changes in the intracellular calcium concentration were monitored by ratio-fluorimetry (T.I.L.L. Photonics, Martinsried). Several experiments were done after pre-incubation of the cells with appropriate blocking agents and thereafter stimulation in the presence of the blocking agents to further characterize the involved transmitter receptors.
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(1998). Calcium Imaging Of Smooth Muscle Cells Cultured From Different Regions OF Human And Guinea Pig Lower Urinary Tract.. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/pharmtox/neuhaus0582/index.html