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Reproductive Toxicity of Cadmium

Contact Person: Peter Massanyi (massanyi@afnet.uniag.sk)

Materials and Methods

Concentrations of cadmium in non-exposed cattle (16) were determinated by atomic absorption spectrophotometry (AAS Perkin Elmer mod. 760, Unicam Solar mod. 929) using the aspiration method (1), of the ash solution with 1% HNO3, and then read at 228,8 Ám wavelenght, following the Unicam AAS methods manual. Histological experiments were conducted on rabbits (Hyla, RIAP Nitra). Animals (56) were divided into 4 groups (K,A,B,C). Eight rabbits received cadmium i.p. (1,5 mg Cd/kg body weight). These animals (group A) were killed 48 hours after application of cadmium (CdCl2, Sigma, St.Louis, MO). Chronic experiment (group B) was carried out on the same number of animals. Cadmium was administrated at 1,0 mg Cd/kg b.w. daily for 5 month in pelletized food.

The same dose was administered to animals which were mated after experimental period (Group C). Group K was the control, receiving no cadmium. Ovaries, oviducti, uteri, testes and epidydimis from all animals were proceeded by routine histological methods and analyzed by micromorphiological criteria (5,6,7). Also AAS analyses were used for cadmium level detection. Porcine granulosa cells were cultured in the medium with addition of cadmium (CdCl2) at three doses (0,2; 10; 20 ng/ml - D,E,F). Control medium (K) contained no cadmium. After 48 hours of culture granulosa cells were proceeded for ultrastructure analysis (TEM) and medium was analyzed for progesterone (P) and 17-beta oestradiol (E) by RIA methods (8,9). Cadmium was added into the thawed semen samples in four concentrations (0,02; 0,1; 0,2; 2,0 mg/ml of Natrium citrate - G,H,I,J). Motility parameters of spermatozoa were analyzed by Hamilton Thorn Motility Analyzer vers. 7. From final data, basic statistical characteristic and analyses of variance by F-test, Scheffe`s test were calculated.

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Massanyi, P; Uhrin, V; Toman, R; Lukac, N; Pizzi, F; Renon, P; Paksy, K; Paksy, Z S; Trandzik, J; (1998). Reproductive Toxicity of Cadmium. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/occupational/massanyi0161/index.html
© 1998 Author(s) Hold Copyright