Cancer Poster Session
Dotzlaw, H (Dept.Biochem. and Mol. Bio., University of Manitoba, Canada)
Watson, PH (Dept.Pathology, University of Manitoba, Canada)
Murphy, LC (Dept.Biochem. and Mol. Bio., University of Manitoba, Canada)
Several variant forms of the estrogen receptor alpha (ER-alpha) mRNA have been described in human breast tissues. Among these, ER-alpha mRNA variants truncated after sequences encoding exon 2 of the wild-type ER-alpha mRNA (ERC4 mRNA), or deleted in exon 3 (ERD3 mRNA) or exon 5 (ERD5 mRNA) sequences, were previously shown to be differentially expressed between independent normal and breast tumor tissues. Using semi-quantitative reverse transcription-polymerase chain reaction assays, we have investigated the expression of these variant mRNAs relative to wild-type ER-a mRNA in 18 samples of normal breast tissues and their adjacent matched breast tumor tissues. A general trend towards a higher ERC4 mRNA and a lower ERD3 mRNA relative expression in the tumor compartment was observed. These differences reached statistical significance when considering only the ER-positive/progesterone receptor positive (p=0.019) and the ER-positive (p=0.023) subsets, as measured by the ligand binding assay, respectively. A significantly (p=0.035) higher ERD5 mRNA relative expression was observed in tumor components overall. These data confirm previous observations and demonstrate that changes in the expression of ER-alpha variant mRNAs relative to wild-type occur between adjacent normal and neoplastic breast tissues.
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|Leygue, E; Dotzlaw, H; Watson, PH; Murphy, LC; (1998). Altered Expression of Estrogen Receptor Alpha Variant mRNAs Between Adjacent Normal Breast And Breast Tumor Tissues.. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/cancer/leygue0756/index.html|
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