Invited Symposium: SERCA-Type of Calcium Pumps and Phospholamban
Dode, L (Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Belgium)
Mountian, I (Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Belgium)
Raeymaekers, L (Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Belgium)
In vertebrates, the transcription of three distinct genes (SERCA1, 2, and 3) and alternative processing of the 3'-end of their pre-mRNA contribute to the isoform diversity of the SERCA family. Our recent studies concerning the expression and characterization of the SERCA3 gene provided the following insights: I) SERCA3 and SERCA2b are co-expressed both at protein and mRNA level in certain cell types and cell lines from human and rat (Wuytack et al., 1994, J. Biol. Chem., 269, 1410-1416). II) Human SERCA3 cDNA and genomic clones were isolated and the gene (ATP2A3) was mapped to human chromosomal position 17p13.3 (Dode et al., 1996, Biochem. J., 318, 689-699). III) The human SERCA3 gene (50 kb) consists of 22 exons and its transcription is driven by a GC-rich TATA-less promoter (the promoter region from -135 to -31, containing six putative Sp1 sites, is critical for the transcription of SERCA3 in Jurkat cells); SERCA3a, 3b, and 3c isoforms presenting different C-termini are generated, respectively, by exclusion, partial and total retention of exon 21 in both human and mouse genes. Mouse SERCA3 isoforms overexpressed in COS cells are able to function as active pumps, but with somewhat different affinities for Ca2+ (SERCA3a > SERCA3b and SERCA3c) (Dode et al., 1998, 273, 13982-13994).
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|Wuytack, F; Dode, L; Mountian, I; Raeymaekers, L; (1998). SERCA3 Calcium Transport ATPase Isoform Diversity. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/wuytack/wuytack0580/index.html|
|© 1998 Author(s) Hold Copyright|