Ophthalmology Poster Session
Perkins, BD. (Dept. of Biochemistry, Baylor College of Medicine, USA)
Wensel, TG. (Dept. of Biochemistry, Baylor College of Medicine, USA)
Wilson, JH. (Dept. of Biochemistry, Baylor College of Medicine, USA)
Autosomal dominant retinitis pigmentosa (ADRP) is a progressive disease, that originates in the photoreceptor cells and ultimately causes degeneration of the entire retina. Although mutations in several genes can lead to ADRP, the most common mutations affect the gene rhodopsin, which encodes a G-protein-linked receptor critical for the visual response. As a first step in exploring oligonucleotide-based approaches toward gene therapy for ADRP, we have created cell lines that express rhodopsin or rhodopsin-GFP from a genomic copy of the human gene, which is normally expressed in rod cells. Using various techniques ( Southern blotting, RT-PCR, Western blotting, fluorescence and immunofluorescence) we have demonstrated rhodopsin expression in these cell lines and shown that it is present in the plasma cell membrane.
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|Intody, Z.; Perkins, BD.; Wensel, TG.; Wilson, JH.; (1998). Development of Human Cell Lines Expressing Human Rhodopsin and Rhodopsin-GFP. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/ophthalmology/intody0490/index.html|
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