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Signaling Mechanisms Involved in Endothelial Cell Activation by Perturbed Flow

Contact Person: Peter I Lelkes (pilelkes@facstaff.wisc.edu)


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Kanda, K., Hayman, G.T., Silverman, M.D., and Lelkes, P.I. (1998). Comparison of ICAM-1 and VCAM-1 expression in various human endothelial cell types and smooth muscle cells. Endothelium,6,33 -44.

Kettlun C.S, Samet, M.M. and Lelkes, P.I. (1996) Pulsatile, disturbed flow enhances VCAM-1 expression in cultured human endothelial cells, J. Vasc. Res. 33, S49.

Khachigian, L.M., Resnick, N., Gimbrone, M.A., Jr., and Collins, T. (1995). Nuclear factor-kappa B interacts functionally with the platelet-derived growth factor B-chain shear-stress response element in vascular endothelial cells exposed to fluid shear stress. J Clin Invest, 96, 1169-1175.

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Khachigian, L.M., Lindner, V., Williams, A.J., and Collins, T. (1996). Egr-1-induced endothelial gene expression: a common theme in vascular injury. Science, 271, 1427-1431.

Khachigian, L.M., Williams, A.J., and Collins, T. (1995). Interplay of Sp1 and Egr-1 in the proximal plat elet-derived growth factor A-chain promoter in cultured vascular endothelial cells. J Biol Chem, 270, 27679-27686.

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Lelkes, P.I., ed. (1999) Mechanical Forces and the Endothelium, Harwood Academic Publishers, London, U.K. in press

Lelkes, P.I., and Samet, M.M. (1991). Pulsatile flow and EC morphology in a VAD-like chamber. ASAIO J, 37, M315-M316.

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Nikolaychik, V.V., Samet, M.M., and Lelkes, P.I. (1994). A new, cryoprecipitate-based coating for improved endothelial cell attachment and growth on medical grade artificial surfaces. ASAIO J, 40, M846-M852.

Papadimit riou, E., and Lelkes, P.I. (1993). Measurement of cell numbers in microtiter culture plates using the fluorescent dye Hoechst 33258. J Immunol Methods, 162, 41-45.

Rasouly, D., Shavit, D., Zuniga, R., Elejalde, R.B., Unsworth, B.R., Yayon, A., Lazarovici, P., and Lelkes, P.I. (1996). Staurosporine induces neurite outgrowth in neuronal hybrids (PC12EN) lacking NGF receptors. J Cell Biochemistry, 62, 356-371.

Read, M.A., Neish, A.S., Gerritsen, M.E., and Collins, T. (1996). Postinduction transcriptional repression of E-selectin and vascular cell adhesion molecule-1. J Immun, 157, 3472-3479.

Read, M.A., Whitley, M.Z., Williams, A.J., and Collins, T. (1994). NF-kappaB and IkappaBalpha: an inducible regulatory system in endothelial activation. J Exp Med, 179, 503-512.

Resnick, N., Collins, T., Atkinson, W., Bonthron, B.T., Dewey, C.F., and Gimbrone, M.A., Jr. (1993). Platelet-derived growth factor B chain promoter contains a cis-acting fluid shear-stress-responsive element. Proc Natl Acad Sci USA, 90, 4591 -4595.

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Samet, M.M., and Lelkes, P.I. (1993). Flow patterns and endothelial cell morphology in a simplified model of an artificial ventricle. Cell Biophys, 23, 139-163.

Samet, M.M., and Lelkes, P.I. (1994). Pulsatile flow and endothelial cell morphology in a cell culture chamber model of an artificial cardiac ventricle. J Biomech Eng, 116, 369-371.

Samet, M.M., and Lelkes, P.I. (1999) The hemodynamic environment of the endothelium in vivo and it simulation in vitro IN: Mechanical forces and the endothelium (Lelkes, P.I., ed.), Harwood Academic Press, London, U.K., in press.

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Shyy, J.Y.-J., Li, Y.-S., Lin, M.-C., Chen, W., Yuan, S., Usami, S., and Chien, S. (1995). Multiple cis-elements mediate shear stress-induced gene expression. J Biomechanics, 28, 1451-1457.

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Silverman, M.D., Manolopoulos, V.G., Unsworth, B.R., and Lelkes, P.I. (1996). Tissue factor expression is differentially modulated by cyclic mechanical strain in various human endothelial cells. Blood Coagul Fibrinolysis, 7, 281-288.

Sumpio, B.E.(ed.). (1993). Hemodynamic forces and vascular cell biology. Austin: R. G. Landes Company.

Sumpio, B.E., Du, W., Galagher, G., Wang, X.-J., Khachigian, L.M., Collins, T., and Gimbrone, M.A.,Jr. (1998) Regulation of PDGF-B in endothelial cells exposed to cyclic strain. Arterioscler. Thromb. Vasc. Biol. 18, 349-355.

Takahashi, M., and Berk, B.C. (1996). Mitogen-activated protein kinase (ERK1/2) activation by shear stress and adhesio n in endothelial cells - Essential role for a herbimycin-sensitive kinase. J Clin Invest, 98, 2623-2631.

Wung, B.S., Cheng, J.J., Hsieh, H.J., Shyy, Y.J., and Wang, D.L. (1997). Cyclic strain-induced monocyte chemotactic protein-1 gene expression in endothelial cells involves reactive oxygen species activation of activator protein 1. Circ Res, 81, 1-7.

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Lelkes, P.I.; Kettlun, C.S.; Wigboldus, J.; Waters, C.R.; Sukovich, D.A.; Rubanyi, G.M.; (1998). Signaling Mechanisms Involved in Endothelial Cell Activation by Perturbed Flow. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/
© 1998 Author(s) Hold Copyright