Neuropharmacology Poster Session
Palucha, A (Insitute of Pharmacology, Polish Academy of Science, Poland)
Pilc, A (Insitute of Pharmacology, Polish Academy of Science, Poland)
The phosphorylation of substrate proteins by protein kinases play a key role in signal transduction and function of neurons. Protein kinases has been associated with several physiological and pathological states including depression. It has been observed that a chronic treatment of rats with antidepressants inluences the activity of cAMP-dependent protein kinase (PKA) as well as protein kinase C (PKC) in soluble and particulate fractions from rat brain cerebral cortex and hippocampus. The aim of the present study was to investigate the effect of antidepressants and electroconvulsive treatment on the activity of calcium/calmodulin dependent protein kinase II (CaM-KII) in the hippocampus. Methods: Male Wistar rats were given 7 electroshocks (ECS) delivered 7 times through ear clip electrodes or 14 daily injections of imipramine (10 mg/kg i.p.). CaM-KII activity was measured by the phosphorylation of hydroxyl groups of serine and threonine residues of synthetic substrate AK III by 32P ATP). Results: Our results indicate that repeated (but not single) imipramine administration significantly decreased CaMKII activity in the hippocampus (soluble fraction), a prolonged ECS produced an identical effect. The single and repeated administration of imipramine induced a significant increase in the activity of CaM-KII in particulate fraction from hippocampus, the effect was much more pronounced after repeated administration of imipramine. Discussion: Imipramine or ECS treatment causes a pronounced changes in the activity of CaM-KII in hippocampus, the activity of the enzyme in soluble fraction decreases while the activity of the enzyme in particulate fraction goes up. It is possible that changes in the enzyme activity may be of importance in the mechanism of action of antidepressant drugs. Supported by KBN grant No 4 P05 A003.09.
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|Branski, P; Palucha, A; Pilc, A; (1998). Calcium/ Calmodulin-Dependent Protein Kinase Activity In Rat Brain Is Influenced By Antidepressant Treatment. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/neuropharm/branski0332/index.html|
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