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Immunology & Immunological Disorders Poster Session






Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References




Discussion
Board

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Cytotoxicity of EDTA Used in Biological Samples: Effect on Some Breast-milk Studies


Contact Person: Michael O. Ogundele (mogundel@yahoo.com)


References

1. Chappell JE; Clandinin MT; McVey MA; Chance GW Free fatty acid content of human milk: physiologic significance and artifactual determinants. Lipids. 20(4): 216-21 (1985)

2. Brownie CF; Brownie C; Noden D; Krook L; Haluska M; Aronson AL Teratogenic effect of calcium edetate (CaEDTA) in rats and the protective effect of zinc. Toxicol Appl Pharmacol. 82(3): 426-43 (1986)

3. Rosenblatt D.E., Doyle D:G., Aronson A.L. Calcium ethylenediaminetetraacetate (Ca EDTA) toxicity: time- and dose-dependent studies on intestinal morphology in the rat. Exp. Mol. Pathol. 28(2):215-26 (1978)

4. Heindorff K; Aurich O; Michaelis A; Rieger R Genetic toxicology of ethylenediaminetetraacetic acid (EDTA). Mutat Res. 115(2): 149-73 (1983)

5. Tucker JD; Christensen ML Effects of anticoagulants upon sister-chromatid exchanges, cell-cycle kinetics, and mitotic index in human peripheral lymphocytes. Mutat Res. 190(3): 225-8 (1987)

6. Singh K; Joshi L. Interaction of EDTA with tributyltin induced cellular toxicity. Indian J Exp Biol. 27(9): 833-4 (1989)

7. Samuelson KJ; Rupnow JH; Froning GW The effect of lysozyme and ethylenediaminetetraacetic acid on Salmonella on broiler parts. Poult Sci. 64(8): 1488-90 (1985)

8. Grass GM; Wood RW; Robinson JR Effects of calcium chelating agents on corneal permeability. Invest Ophthalmol Vis Sci. 26(1): 110-3 (1985)

9. Crago SS; Prince SJ; Pretlow TG; McGhee JR; Mestecky J Human colostral cells. I. Separation and characterization. Clin Exp Immunol. 38(3): 585-97 (1979)

10. Buchheim W; Welsch U; Huston GE; Patton S Glycoprotein filament removal from human milk fat globules by heat treatment. Pediatrics. 81(1): 141-6 (1988)

11. Lewis-Jones-DI; Lewis-Jones-MS; Connolly-RC; Lloyd-DC; West-CR. Sequential changes in the antimicrobial protein concentrations in human milk during lactation and its relevance to banked human milk. Pediatr-Res. 19(6): 561-5 (1985)

12. Mayer M.M. Complement and Complement fixation. In Experimental Immunochemistry (2nd edition) Kabat E.A., Mayer M.M., editors. Charles C. Thomas, Publisher, Springfield, IL pp. 133-240 (1971)

13. Bradford-MM A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal-Biochem. 72: 248-54 (1976)

14. Kane-MH; Rodman-JS; Horten-B; Reckler-J; Marion-D; Vaughan-ED Jr Urothelial injury from ethylenediaminetetraacetic acid used as an irrigant in the urinary tract.J-Urol. 142(5): 1359-60 (1989)

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<= Discussion & Conclussions REFERENCES

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Ogundele, M.O.; (1998). Cytotoxicity of EDTA Used in Biological Samples: Effect on Some Breast-milk Studies. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/immunology/ogundele0175/index.html
© 1998 Author(s) Hold Copyright