Invited Symposium: Na-H Exchangers and Intracellular pH Regulation



Materials & Methods


Discussion & Conclusion



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NHE Proton Modifier Site: Activation and Inactivation Are Controlled by Slow Protein Conformational Changes

Contact Person: James L. Kinsella (kinsellj@grc.nia.nih.gov)


  1. Otsu K, Kinsella, J., Sacktor, B., and Froehlich, J.P. (1989) Transient state kinetic evidence for an oligomer in the mechanism of Na+-H+ exchange. Proc Natl Acad Sci U.S.A. 86: 4818-4822.

  2. Otsu, K, Kinsella, J.L., Koh, E., and Froehlich, J.P. (1992) Proton dependence of the partial reactions of the sodium-proton exchanger in renal brush border membranes. J. Biol. Chem. 267(12): 8089-8096.

  3. Otsu, K., Kinsella, J. L., Heller, P., and Froehlich, J P. (1993) Sodium dependence of the sodium proton exchanger in the pre-steady state: Implications for the exchanger mechanism. J. Biol. Chem. 268: 3184-3193.

  4. Aronson, P. S., Nee, J., and Suhm, M.A. (1982) Modifier role of internal H+ in activating Na+/H+ exchanger in renal microvillus membrane vesicles. Nature Lond. 299: 161-163.

  5. Kinsella, J.L. and Aronson, P.S. (1980) Properties of the Na+-H+ exchanger in renal microvillus membrane vesicles. Am. J. Physiol. 238: F461-F469.

  6. Warnock, D.G., Reenstra, W.W., and Yee, V.J. (1982) Na+/H+ antiporter of brush border vesicles: studies with acridine orange uptake. Am. J. Physiol. 242: F733-F739.

  7. Fliegel, L., Haworth, R. S., Dyck, J. R. B. (1993) Characterization of the placental brush border membrane sodium proton exchanger identification of thiol-dependent transitions in apparent molecular size. Biochem. J. 289: 101-107.

  8. Amemiya, M., Loffing, J., Loetscher , M., Kaissling, B., Alpern, R.J., and Moe, O.W. (1995) Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb. Kidney Int. 48: 1206-1215.

  9. Fafournoux, P., Noel, J., and Pouyssegur, J. (1994) Evidence that Na+-H+ exchanger isoforms NHE1and NHE3 exist as stable dimers in membranes with a high degree of specificity for homodimers. J. Biol. Chem. 269: 2589-2596.

  10. Orlowski, J. and Grinstein, S. (1997) Na+/H+ exchangers of mammalian cells. J. Biol. Chem. 272: 22373-22376.

  11. Noel, J. and Pouyssegur, J. (1995) Hormone regulation, pharmacology, and membrane sorting of vertebrate Na+/H+ exchanger isoforms. Am. J. Physiol. 268: C283-C296.

  12. Weinman, E.J. and Shenolikar, S. (1993) Regulation of the renal brush border membrane Na+-H+ exchanger. Ann. Rev. Physiol. 55: 289-304.

  13. Hoogerwerf, W.A., Tsao, S.C., Devuyst, O., Levine, S.A., Yun, C.N.C., Yip, J.W., Cohen, M.E., Wilson, P.D., Lazenby, A.J., Tse, C.M., and Donowitz, M. (1996) NHE2 and NHE3 are human and rabbit intestinal brush-border proteins. Am. J. Physiol. 270: G29-G41.

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Kinsella, J; Froehlich, J; (1998). NHE Proton Modifier Site: Activation and Inactivation Are Controlled by Slow Protein Conformational Changes. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/fliegel/kinsella0254/index.html
© 1998 Author(s) Hold Copyright