Cell Biology Poster Session
Staining for neutrophil elastase was observed on numerous cells in regions of necrosis and around some of the maternal blood vessels [Figure 1b]. Some cells in the regions of necrosis were also positive for leucocyte common antigen. CD56 positive natural killer cells and HAM-56 positive macrophages were scattered around the decidual stroma and in close apposition with the interstitial trophoblast. The interstitial trophoblast cells were nonreactive with anti-CD56, anti-HAM-56 and anti-leucocyte common antigen. No significant number of neutrophils labelled with anti-neutrophil elastase was observed in areas of trophoblast invasion. No labelling was observed on negative control sections where the primary antibody was replaced by a non-immune serum.
Fig. 1a: Photomicrograph of first trimester human decidua basalis immunolabelled with mouse monoclonal anti-cytokeratin. The reaction product of DAB is seen on the interstitial trophoblast cells (arrow) in the decidual stroma (D). The maternal leucocytes present in the region of necrosis are unreactive (X). Maternal blood vessels (V).
(Mag: x130, Bar: 100 um).
Fig. 1b: An adjacent section immunolabelled with anti-neutrophil elastase. The reaction product of New Fuchsin is confined to the neutrophils in the region of necrosis (X) and around some of the maternal blood vessels (V). Decidual stroma (D).
(Mag: x130, Bar: 100 um).
Fig. 2a: Electron micrograph of the first trimester human decidua basalis showing the two cell types. A decidual stromal cell (D) with a single nuclear profile, a prominent nucleolus and an electron lucent cytoplasm. The other two cells are type I agranular interstitial trophoblasts (T). They have an electron dense cytoplasm, a single large convoluted nuclear profile (n) and numerous cell processes (arrowhead). (Mag: x6410, Bar: 5 um).
Fig. 2b: The type II granular interstitial trophoblast cells. The cells have numerous outer cell processes (P). There is extensive degenerative cellular material in the extracellular space (ec). There are also a few cells in this area with features suggestive of apoptosis (A) or secondary necrosis (S).(Mag: x1941, Bar: 10 um).
Fig.2c: A higher magnification showing several of the type II granular interstitial trophoblast cells. Their cytoplasm is electron dense and contains many granules.
Fig. 3: An electron micrograph showing the type I agranular interstitial trophoblast with similar ultrastructural morphology to the cells shown in figure 17a. This cell has a large convoluted nuclear profile (n) and a prominent nucleolus. The cytoplasm contains much granular endoplasmic reticulum (er). Cell membranes have prominant processes (P). Cellular debris may be seen in the extracellular space (ec). An adjacent decidual stromal cell (D) is also present.
(Mag: x14040, Bar: 2 um).
Fig. 4 : A high magnification micrograph showing the cytoplasmic details of the type I agranular interstitial trophoblast cell. A cluster of glycogen (g), mitochondria (m), Golgi apparatus (G) and secretory granule (s). Nucleus (n).(Mag: x27791, Bar: 1 um).
Fig. 5. A type I agranular interstitial trophoblast cell from tissue embedded in Lowicryl HM 20 and immunolabelled with mouse anti-cytokeratin followed by streptavidin-1 nm gold particles. A significant number of gold particles are present on the bundles of filaments in the cytoplasm (arrowhead). Intracellular space (i); Nucleus (n).
(Mag: x27791, Bar: 1 um).
Fig.6a. Electron micrograph of a type II granular interstitial trophoblast cell immunolabelled for cytokeratin. Silver enhanced gold particles are seen over the cytoplasm over individual filaments.
(Mag: x17500, Bar: 2 um).
Fig.6b: A higher magnification of another type II cell. Numerous gold particles (arrowhead) are seen over the cytoplasm, bundles of filaments are not seen.(Mag: x21312,Bar: 1 um).
Fig.6c. Electron micrograph from a section immunolabelled for anti-human placental lactogen followed by streptavidin/1 nm gold particles and silver enhancement. Numerous gold particles (arrowhead) are present on the cytoplasmic granules of this type II granular interstitial trophoblast cell.(Mag: x40,300; Bar: 1 um).
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|Al-Lamki, R.S; Skepper, J.N; Wooding, P.B.P; Burton, G.J; (1998). The Interstitial Trophoblast of the First Trimester Human Decidua Basalis Is Composed of Two Distinct Cell Types: an Ultrastructural and Immunocytochemical Study. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/cellbio/al-lamki0114/index.html|
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