Invited Symposium: Behaviour-Induced Neural Events after Brain Injury
Materials and Methods
- 40 adult (3-4 Ĺ months) male Long-Evans rats were placed into one of the 4 following groups: 1) Corpus Callosum Transections + Limb-Restricting Cast (CX + Cast; Fig. 1) 2) Corpus Callosum Transections + Control Cast (CX + Control Cast; Fig. 2) 3) Sham + Limb-Restricting Cast (Sham + Cast) 4) Sham + Control Cast - Corpus Callosum Transections (CX)of the rostral 2/3rdís of the corpus callosum (Fig. 3)were performed using a moving insulated platinum electrode with exposed tip (0.5 mA for 15s). - Immediately following surgery, animals were placed into fiberglass casts. - After 18 day survival, animals were perfused and brains were impregnated with Golgi-Cox. - Brains were resin embedded and Layer V basilar dendrites (Fig. 4)of the forelimb sensory motor cortex (FLsmc) were analyzed in the hemisphere opposite the side of approach of the lesion and opposite the side of the forced-use limb.Experiment 2
- Adult Long-Evans rats were divided into four groups: 1) lesion rats trained on the acrobatic task (Lesion-AC; Fig. 7) 2) lesion rats given simple exercise as a motor control condition (Lesion-MC; Fig. 8) 3) sham-operated rats trained on the acrobatic task (Sham-AC) 4) sham operated motor control rats (Sham-MC) - The lesion groups were anesthetized and given an electrolytic lesion of the forelimb representation area of the sensorimotor cortex (FLsmc, Fig. 9). - Training on the acrobatic task began on the second day after the lesion and continued until day 28. - Following perfusion, the cerebelli were resin-embedded and processed for light and electron microscopy. - For light microscopy, a series of semi-thin sections of 1 µm, separated by 4 µm gaps, were Nissl stained (Fig. 12). Sections were analyzed for Purkinje cell body volume and for density of Purkinje cells, using the physical disector method(see Results). - Electron micrographs were taken of sequential 70 nm sections in the outer 1/3 of the cerebellar molecular layer. Micrographs were analyzed for synaptic density (Fig. 11)using the physical disector method.
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|Bury, S; Grande, L; Ishida, J; Zitzka, A; Jones, T; (1998). The Effects Of Behavioral Demand On Motor Cortical And Cerebellar Structural Plasticity After Brain Injury In Adult Rats. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/schallert/bury0827/index.html|
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