Neuropharmacology Poster Session
Quinpirole (LY171555) is a selective D2-like receptor agonist used extensively in vivo and in vitro. [3H]Quinpirole binds selectively to D2-like receptors in vitro (Levant et al., JPET, 262 (3) 929-935, 1992). Surprisingly, the binding of [3H]quinpirole to D2-like sites in rat striatum is competitively inhibited by the monoamine oxidase inhibitors (MAOIs) Ro 41-1049, clorgyline, (+)-deprenyl and (-)-deprenyl (Levant et al., EJP, 241:141, 1993). In addition, the MAOI clorgyline has been shown to block the development and maintenance of quinpirole-induced behavioral sensitization (Culver and Szechtman, EJP, 339:109-111, 1997) suggesting that MAOI interactions at quinpirole labeled sites have functional importance.
The function of G protein-coupled receptors, which include D2-like receptors, can be assessed by agonist-stimulated [35S]GTPgammaS binding in crude membrane preparations. This provides a measure of G protein activation, the initial step in signal transduction.
The PURPOSES of these studies were to: 1) characterize quinpirole-stimulated [35S]GTPgammaS binding in rat striatal membranes, and 2) determine if MAOIs modulate quinpirole-stimulated [35S]GTPgammaS binding.
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|Gilliland, S.; Alper, R.; Levant, B.; (1998). Characterization of Quinpirole-Stimulated [35S] GTPgammaS Binding at D2-like Receptors in Rat Striatum.. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/neuropharm/gilliland0401/index.html|
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