Pharmacology & Toxicology Poster Session

Re: Poster 665

Albert Woelfler

On Sun Dec 6, Grover wrote
>Dr. Woelfler: Great presentation. áHope you have fun at the meeting. áI had never thought of melatonin as a calmodulin antagonist and your results show that it is not. áI am not sure what concentrations of TFP you used as TFP itself can have membrane effets unrelated to calmodulin.

Dear Dr Grover,

Melatonin has been reported to bind to the calcium/calmodulin complex with high affinity (Life Sci (1993)53: 201-207; FASEB (1998) 13: 1401-1408) and it has been shown to inhibit Ca/calmodulin -dependent enzymes in presence of Ca/calmodulin in biochemical assays (Brain Res (1991) 557: 289-292; Biochim Biophys Acta (1996) 1290: 191-196). However, whether melatonin can bind to calmodulin and inhibit the activation of calmodulin-dependent enzymes within cells is ánot known.
We used 1 to 10ÁM TFP. At this concentrations TFP has been shown to be a potent calmodulin inhibitor.

Albert Woelfler

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