Pineal and its Hormone
Re: SCN cells capable of c-Fos photoinduction
Dear Dr. Pang,
thank you for your questions. Using the standard immunocytochemistry, we can only distinguish cells producing c-Fos protein as dark dots above the background staining. This tells us how many cells in a section respond by production of c-Fos protein as a function of time. The other population of cells - non responding to light - would need to be visualized by any of the commonly used counterstaining methods and counted in addition to the ICC stained cells.
All the best,
Mon Dec 14