Kenneth B. Storey
When does % identity decrease to a point where we are talking about a new isoform? You indicate % identities of 90 percent and over in your comparisons! is 90% enough in a species match between pigs and rodents? would it be enough for human vs bovine? Is 90% identity correspondent to the SAME isoform, or at this level are we talking about another member of a gene family?
Many labs are using BLAST to find genes that they have identified in non-mammalian species - how far can % identities help us in determining homologous sequences?