Thank you for your comment and question.
Identification of spot proteins by mass spectrometry is now ongoing in our laboratory. But we have not yet succeeded in assigning any spot on the 2-D gel map to proteins which have been reported as a cell senescence marker. We may achieve direct identification of spot proteins if the specific antibody is available. But unfortunately we don't have such antibody. So I would like to ask for collaboration with researchers who may offer specific antibodies against cellular senescence-related proteins to assign the location on our 2-D gel map.
We have a plan to compare our 2-D gel map with keratinocyte database prepared by Celis in Danish Centre of Human Genome Research. Common denominators will be applied to matched proteins between two databases.