Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References

Discussion
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EDTA failed to cause any degree of hemolysis of rabbit red blood cells (RRBC) in the absence of additional calcium and magnesium cations, using GVB2- buffer. Mg2+ caused a dose-dependent enhancement of EDTA cytotoxicity, at all concentrations tested, up till 50mM. Ca2+ caused a dose-dependent increase in EDTA-toxicity up to a maximal level of 5mM. Thereafter it caused a progressive inhibition of the EDTA-induced hemolysis in a dose-dependent manner, gradually approaching that of the buffer level

Figure 1: Effect of calcium and magnesium cations on EDTA toxicity in *GVB++ (using unsensitized rabbit erythrocytes as target cells) * GVB2- was used to assess the toxicity of EDTA without any cations

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