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Oxidative Stress Poster Session






Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References




Discussion
Board

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Purification And Characterization Of A Camp-Dependent Protein Kinase >From The Tail Muscle Of The Crayfish, Orconectes Virilis.


Contact Person: Dr. Kyra J. Cowan (kcowan@corr.com)


References

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Bishoff, S T, Combest, W L, Gilbert, L I (1990) Characterization of cAMP-dependent protein kinase from the pupal wing epidermis of Manduca sexta. Insect Biochem. 20: 397-404

Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 12: 248-253.

Brooks, S.P.J. (1992) A simple computer program with statistical tests for the analysis of enzyme kinetics. Bio-Techniques. 13: 906-911.

Brooks, S P J, Storey, K B (1990) cGMP-stimulated protein kinase phosphorylates pyruvate kinase in an anoxia-tolerant marine mollusc. J Comp Physiol B 160: 309-316

Brooks, S P J, Storey, K B (1994) Patterns of protein synthesis and phosphorylation during anoxia in the land snail Otala lactea. Can. J. Zool. 72: 856-862

Brooks, S P J, Storey, K B (1996) Protein kinase involvement in land snail aestivation and anoxia: Protein kinase A kinetic properties and changes in second messenger compounds during depressed metabolism. Mol. Cell. Biochem. 156: 153-161

Cao, J, Fernandez, M, Vazquez-Illanes, M D, Martinez, J I R, Villamarin, J A (1995) Purification and characterization of the catalytic subunit of cAMP-dependent protein kinase from the bivalve mollusc Mytilus galloprovincialis. Comp. Biochem. Physiol. B 111: 453-462

Cao, J, Ramos-Martinez, I, Villamarin, J A (1995) Characterization of a cAMP-binding protein from the bivalve mollusc Mytilus galloprovincialis. Eur. J. Biochem. 232: 664-670

Carr, D W, Scott, J D (1992) Blotting and band-shifting: techniques for studying protein-protein interactions. Trends Biochem. Sci. 17: 246-249

Combest, W L, Gilbert, L I (1989) Particulate associated cAMP-dependent protein kinase in the brain of the tobacco hornworm, Manduca sexta. Insect Biochem. 19: 663-672

Cowan, K J, Storey, K B (1999) Reversible phosphorylation control of glycolysis in the anoxia tolerant crayfish, Orconectes virilis, submitted.

Fernandez, M, Cao, J, Dolores, M, Ramos-Martinez, J I, Villamarin, J A (1994) Phosphofructokinase from mantle tissue of Mytilus galloprovincialis: purification and effects of phosphorylation on the enzymatic activity. Biochem. Mol. Biol. Int. 33: 355-364

Hochachka, P W, Somero, G N (1984) Biochemical adaptation. Princeton University Press, Princeton, N J.

Holden, C P , Storey, K B (1997) Signal transduction, second messenger and protein kinase responses during freezing exposures in the wood frog. Am. J. Physiol. 271: R1205-1211.

Holwerda, D A, Veenhof, P R, van Heugten, H A A, Zandee, D I (1983) Regulation of mussel pyruvate kinase during anaerobiosis and in temperature acclimation by covalent modification. Mol. Physiol 3: 225-234

Jiang, H. and Corbin, J.D. (1991) Effect of epinephrine or cAMP on cAMP-bound protein kinase holoenzymes in rat heart. Am. J. Physiol. 260: H722-H729.

Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685.

Mehrani, H, Storey, K B (1995) cAMP-dependent protein kinase and anoxia survival in turtles: purification and properties of liver PKA. Mol. Cell. Biochem. 145: 81-88

Michaelidis, B, Storey, K B (1990) Phosphofructokinase from the anterior byssus retractor muscle of Mytilus edulis: modification of the enzyme in anoxia and by endogenous protein kinases. Int. J. Biochem. 22:759-765

Roskoski Jr., R. (1983) Assays of protein kinase. Meth. Enzymol. 99: 3-6.

Scott, J.D., Bergeson, S.E., Langeberg, L., Nilaver, G., Coghlan, V.M. (1993) A-Kinase anchoring protein - a key to selective activation of cAMP-responsive elements. Mol. Cell. Biochem. 128: 309-319

Scott, J D, Carr, D W (1992) Subcellular localization of the Type II cAMP-dependent protein kinase. News Physiol. Sci. 7: 143-148

Storey, K B (1984) Phosphofructokinase from the foot muscle of the whelk, Busycotypus canaliculatum: evidence for covalent modification of the enzyme during anaerobiosis. Arch. Biochem. Biophys. 235: 665-672

Storey, K B, Storey, J M (1990) Facultative metabolic rate depression: molecular regulation and biochemical adaptation in anaerobiosis, hibernation, and estivation. Quart Rev Biol 65: 145-174.

Sugden, P H, Holladay, L A, Reimann, E M, Corbin, J D (1976) Purification and characterization of the catalytic subunit of adenosine 3:5-cyclic monophosphate-dependent protein kinase from bovine liver. Biochem. J. 159: 409-422

Taylor, S.S., J.A. Beuchler, and D.R. Knighton. (1990) Cyclic AMP-dependent protein kinase: Mechanism for ATP:protein phosphotransfer. In: Peptides and protein phosphorylation. ed. B. Kemp. CRC Press, Boca Raton, FA. pp. 1-41.

Walsh, D A, Newsholme, P, Cawley, K C, van Patten, S M, Angelos, K L (1991) Motifs of protein phosphorylation and mechanisms of reversible covalent regulation. Physiol. Rev. 71:285

Walsh, D A, Van Patten, S M (1994) Multiple pathway signal transduction by the cAMP-dependent protein kinase. FASEB J. 8: 1227-1236

Whitwam, R E, Storey, K B (1990) Regulation of phosphofructokinase during estivation and anoxia in the land snail, Otala lactea. Physiol. Zool. 64: 595-610

Yamamura, H, Nishiyama, K, Shimomura, R, Nishizuka, Y (1973) Comparison of catalytic units of muscle and liver adenosine 3,5-monophosphate dependent protein kinase. Biochemistry 12: 856-863

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<= Discussion & Conclussions REFERENCES

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Cowan, K.J.; Storey, K.B.; (1998). Purification And Characterization Of A Camp-Dependent Protein Kinase >From The Tail Muscle Of The Crayfish, Orconectes Virilis.. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Available at URL http://www.mcmaster.ca/inabis98/oxidative/cowan0409/index.html
© 1998 Author(s) Hold Copyright