The ethanol-sensitive ANT (alcohol-nontolerant) and ethanol-insensitiveAT (alcohol-tolerant) rats have been selectively bred for high and lowsensitivity to motor impairment in a tilting plane test after ethanol.This study was designed to find out if differences exist in the brain histaminergicsystem between naive rats of these lines. Histamine concentrations in thehypothalamus, cerebral cortex, and septum of AT rats were significantlyhigher than those of ANT rats. The counts of histamine-containing mastcells were not different, but the density of histamine-immunoreactive nervefibers in several brain areas was higher in AT rats than in ANT rats. Inboth lines, the expression of L-histidine decarboxylase mRNA was limitedto the tuberomammillary neurons. Expression of histamine H1 receptor mRNAas revealed by quantitative in situ hybridization was lower in severalbrain areas of the AT rats than in ANT rats. No dif ference was observedin expression of H2 receptor mRNA. The results suggest that there are significantdifferences between the two rat lines. Decreased brain histamine levelsin ANT rats are associted with increased expression of the H1 receptor.Future studies are planned to reveal the effects of manipulation of thebrain histamine system on ethanol-induced motor impairment in these rats.
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|Panula, P; Lintunen, M; Kaslin, J; Sallmen,T; Anichtchik, O; Senkas, A; Karlstedt, K; Karlstedt, E; (1998). Alcohol-RelatedBehavior and Brain Histamine: Decreased Brain Histamine and Increased Expressionof H1 Receptor in a Rat Line Sensitive to Motor Impairment by Alcohol.Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciencesat McMaster University, Canada, Dec 7-16th. Invited Symposium. Availableat URL http://www.mcmaster.ca/inabis98/huston/panula0371/index.html|
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