Contact Person: Denis C. Lehotay (email@example.com)
The production of free radicals leads to lipid peroxidation, and is a major contributor to several types of toxic injury. Products of lipid peroxidation play a significant role in aging, drug toxicity, and in the pathophysiology of a number of human diseases such as atherosclerosis, cancer and heart disease. The study of oxidative stress in biological systems requires markers that allow correlations to be established between injury and the products of lipid peroxidation. Our laboratory has developed methods using GC/MS techniques for measuring in-vivo levels of hydroxyl radicals, cytotoxic aldehydes and acyloins (breakdown products of aldehyde metabolism). We used these methods to determine the role of free radicals in the development of oxidative injury in several animal models of disease. Experimental evidence will be presented to support the conclusions below regarding the relationship between free radical production and tissue injury. Our data indicate that free radicals and byproducts of lipid peroxidation can be measured with good precision and accuracy both in plasma and in tissues. Several generalizations can be made about the nature of oxidative injury, and the usefulness of measuring hydroxyl radicals and other reactive oxygen species.
In our experiments apoptotic cell death could be prevented even when significant increases in free radicals occurred. These interventions appear to have blocked steps that were distal to free radical production in the cascade of events leading to cell death. The availability of methods such as those developed by our laboratory has made the study of biochemical changes associated with free radical-mediated tissue injury possible.
Back to the top.
| Discussion Board | Next Page | Your Poster Session |