Molecular and Cellular Analysis of Dopamine and Serotonin Transporters

Re: Two site analysis

Rabi Simantov

To tell you the truth, we didn't.
Using only 7 or 8 points on the uptake curve is very tight for a two-site model, and we therefore prefered to wait for a more complete study (with more points). Yet, from your question I understan that its worth trying; as its an interesting point, we might go back to check it. Thanks.

On Fri Dec 11, Grover wrote
>Dr. Simantov
>Did you try to see if these data would fit better in a two site model?
>On Fri Dec 11, Rabi Simantov wrote
>>Good question. The figure certainly don't rule out this possibility, especially in the TPA-treated cells.
>>To confirm the presence of a such complex uptake system one need to run many more points, possibly 12-14, and we could not do that. It was decided therefore not to conclude from these data anything regarding the kinetics of the uptake, except that TPA increased the uptake by 50-100%.

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