>Is alpha d found on immature eosinophils or on basophils?
>What interaction is there between IL-5R and alpha d expression? Presumably IL-5 would induce alpha d by IL-5R-mediated events.
We did not study the expression of alpha-d on immature cell types; however, it seems reasonable to assume that alpha-d expression is present as these cell types mature, much like CD11b/CD18. Indeed, Van der Vieren, et. al. have shown that the promyelomonocytic cell line HL60 expresses alpha-d and this expression is not affected by DMSO treatment (Immunity. 1995 Dec;3(6):683-90.).
In our initial studies we did not characterize further the induction of alpha-d expression by IL-5. Most likely this effect is mediated through the IL-5R; however, we cannot rule out a more indirect pathway.