Molecular Physiology of Sodium-Calcium Exchange

Re: (0411) A circularized sodium-calcium exchanger exon-2 transcript

Jonathan Lytton

On Tue Dec 8, Paul J. Bauer wrote
>This is very important work. Your idea  that the exon-2-transcript forms a ‘symmetrical exchanger’ by dimerization is interesting. Did you test this hypothesis by cross-linking with SH-specific reagents?

Thanks Paul. No, we haven't done any cross-linking - just run without beta-ME as shown in one of the figures. I think perhaps we should use two different exchanger constructs with different epitope tags, then we could verify cross-linking by immunoprecipitation, or something like that (I know you have used the 2D gel approach).

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