Abstract

Introduction

Materials & Methods

Results

Discussion & Conclusion

References

Discussion
Board

Many phenolic polyamines naturally found in a variety of plants, especially the Solanacea such as tomatoes, show similarities to spider and wasp toxins and may be partially responsible for pest resistance in plants. This study investigates the effects of one of these, N1-coumaroyl spermidine (N1CS), on the synapses of the deep extensor muscles of Procambarus clarkii. N1CS reduced the amplitude of excitatory postsynaptic potentials in a dose dependant manner. The EC50 value for this effect is estimated to be between 0.1mM and 0.03mM. N1CS also blocked responses to iontophoretic application of glutamate in muscle treated with concanavalin A to reduce desensitization. 0.03 mM N1CS blocked these responses by more than 75%, and the effect was completely reversed by washing for about 7 minutes. Thus N1CS blocks glutamate receptors on crayfish muscle reversibly.

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Poster Number PAklose 0724
Keywords: glutamate, receptor, antagonist, spermidine, crustacean