Invited Symposium: Oxidative Stress and the CNS
Yim, H.-S. (Laboratory of Biochemistry, NHLBI, NIH, Bethesda MD, USA)
Chock, P.B. (Laboratory of Biochemistry, NHLBI, NIH, Bethesda MD, USA)
Stadtman, E.R. (Laboratory of Biochemistry, NHLBI, NIH, Bethesda MD, USA)
Familial amyotrophic lateral sclerosis (FALS) is an inherited disorder of motor neurons, which is associated with missense mutations in the Cu,Zn-superoxide dismutase gene. Mice from the G93A transgenic line was reported to develop a syndrome of FALS. The fact that the symptoms occurred against a background of normal mouse Cu,Zn-SOD activity suggests that dominant, gain-of-function mutations in SOD play a role in the pathogenesis of FALS. We investigated the nature of this gain-of-function of FALS mutants. We have previously reported that Cu,Zn-SOD has the free radical generating function in addition to normal dismutation activity. These two enzymic activities were compared by using mutants (G93A and A4V) and the wild-type Cu,Zn-SOD prepared by recombinant method. Our results showed that the wild-type, G93A, and A4V enzymes have identical dismutation activity. However, the free radical generating function of the G93A and A4V mutants, as measured by the spin trapping and EPR method, is enhanced relative to that of the wild-type enzyme (wild-type < G93A < A4V), particularly at lower H2O2 concentrations. This is due to the decrease in the Km value for H2O2, wild-type > G93A > A4V. The catalytic activity to generate free radicals is correlated to the clinical severity of the disorder induced by these mutant enzymes. Furthermore, we found that intact FALS mutants failed to enhance tyrosine nitration. Together, our results indicate that the ALS symptoms are not caused by the reduction of Cu,Zn-SOD dismutation activity with the mutant enzymes; rather, it is induced in part by enhancement of the free radical generating function.
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|Yim, M.B.; Yim, H.-S.; Chock, P.B.; Stadtman, E.R.; (1998). Enhanced Free Radical Generation by FALS-associated Cu,Zn-SOD Mutants. Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/juurlink/yim0183/index.html|
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