Invited Symposium: Molecular Mechanisms of Ageing
Choi, JY (Department of Pharmacology, Seoul National University College of Medicine, Korea)
Lee, DW (Laboratory of Biochemistry, Korea Ginseng and Tobacco Research Institute, Korea)
8-hydroxyguanine(oh8Gua), a premutagenic DNA adduct formed by active oxygens, has been suggested to be relevant to aging. oh8Gua glycosylase (ogg1) removes oh8Gua residues from DNA and prevents the mutation. In the present study, we compared the oh8Gua contents and oh8Gua glycosylase activities in various organs at 2, 8, 14 months of age between senescence-prone strain (SAMP1 and SAMP8) and senescence-resistant strain (SAMR1) of senescence-accelerated mice (SAM; a murine model of accelerated aging). In all the organs of SAMP1 and SAMP8 mice at all ages examined, oh8Gua glycosylase activity was markedly reduced to approximately 10 - 40% of corresponding senescence-resistant SAMR1 mice and oh8Gua content in DNA was much increased. RNA blot analysis revealed no reduction in mRNA levels of ogg1 gene in SAMP mice. We identified a novel R304W point mutation in the mogg1 gene in SAMP mice, which may be responsible for the reduced activity. We propose that the impairment of oh8Gua repair in SAMP mice may be one of the factors contributing to accelerated senescence.
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|Chung, MH; Choi, JY; Lee, DW; (1998). 8-Hydroxyguanine Repair Activity In Aging; Impairment Of 8-Hydroxyguanine Repair In Senescence-Prone Mice (SAMP1 And P8). Presented at INABIS '98 - 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, Dec 7-16th. Invited Symposium. Available at URL http://www.mcmaster.ca/inabis98/higuchi/chung0735/index.html|
|© 1998 Author(s) Hold Copyright|