Molecular and Cellular Analysis of Dopamine and Serotonin Transporters


Re: Poster 727, reply to Dr. Grover

Tiziana Mennini
tiziana@irfmn.mnegri.it


Dear Dr. Grover
First of all we have to apologize for the delay in participating to the discussion board, but our provider was out of order for 4 days and we could reach the web site only yesterday.
As to your interesting question, we said that the d-fenfluramine-induced [3H]5-HT release (fig. 1) was
(5-HT)carrier-dependent because it was completely antagonized by
1 M citalopram, a well known 5-HT carrier blocker.
Citalopram could affect both d-fenfluramine entry into the synaptosome
or the carrier-mediated [3H]5-HT release.
Our data suggest that 5-HT carrier is involved in both mechanisms:
- on d-fenfluramine entry: according to the selectivity of this drug on 5-HT release.
- on [3H]5-HT efflux: since also in the absence of MAO inhibitor the
release is mainly due to unmetabolized 5-HT, as commented in the discussion.
With many thanks, Tiziana Mennini and Marco Gobbi

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