Iron Transport
symposium 473 Kicic/Baker Doug Templeton
doug.templeton@utoronto.ca
Hello Anthony. The MTT assay should give results proportional to cell number if viability is
equivalent. Have you checked viability or cell number by any independent means? Also, I believe
one of the major enzymes involved in MTT reduction is mitochondrial succinate dehydrogenase.
This is an Fe-containing enzyme that can be inhibited by Fe chelators. I wonder if in part your
results reflect accessibility of the chelator to this enzyme?Doug Templeton
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Thu Dec 10