Iron Transport


The Effects of Iron Chelators Upon Cellular, Proliferation and Iron Transport in Hepatoma Cells.

Doug Templeton
doug.templeton@utoronto.ca


Hello Anthony.  The MTT assay should give results proportional to cell number if viability is equivalent.  Have you checked viability or cell number by any independent means?  Also, I believe one of the major enzymes involved in MTT reduction is mitochondrial succinate dehydrogenase.  This is an Fe-containing enzyme that can be inhibited by Fe chelators.  I wonder if in part your results reflect accesibility of the chelator to this enzyme?

Doug Templeton


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