The Effects of Iron Chelators Upon Cellular, Proliferation and Iron Transport in Hepatoma Cells.
Hello Anthony. The MTT assay should give results proportional to cell number if viability is equivalent. Have you checked viability or cell number by any independent means? Also, I believe one of the major enzymes involved in MTT reduction is mitochondrial succinate dehydrogenase. This is an Fe-containing enzyme that can be inhibited by Fe chelators. I wonder if in part your results reflect accesibility of the chelator to this enzyme?
Thu Dec 10