Cell Biology Poster
Re: poster 699
On Fri Dec 4, grover wrote
>Dr. Zador: Hope you are enjoying the meeting. Great poster.After regeneration in the soleus muscle 98% of the fibers express the slow muscle specific SERCA2a isoforms (these are real slow fibers, also expressing the slow myosin heavy chain isoform). However such fibers are only 81% of total in the normal soleus muscle. Two questions. First did the soleus of these rats differ functionally in any way you noticed? Second, what do you attribute this diffrence to or do you even think that if this experiment were allowed to continue for a long duration, this number would come down to 85%?
Dr. Grover: Yes this meeting is great, although apparently I have less time for it than I should. Thank you for organizing it and thank you for your comments and sorry for not answering them immediatelly. Yes we have checked soleus muscles after 5 months of regeneration and the ratio of SERCA2a expressing fibers was not significantly different from the one after four weeks. May be after a longer period of time? We have never attempted to do a functional analysis of the regenerated muscles, but we are planning to try the tetanic stimulation. The increased number of SERCA2a (slow type) fibers of the regenerated soleus is probably due to the fact that this muscle receives domnantly slow innervation.
Tue Dec 15