PHAC CL2 Checklist

A tip for document organization is found here.

Document is to be submitted to the biosafety office after an laboratory audit has been completed and after the supervisor has signed and dated it.

Q1. Our medical monitoring program is found here. You need to have your medical monitoring sheets ready for inspection.

Q2. The current Biological Safety Officer of record is Karin Cassidy.

Q3. A template for a biosecurity plan is found here. Have it ready for inspection.

Q4. Signage should be prominant on all doors leading to the BSL2 laboratory. Authorized access only.

Q5. SOP manual or procedural manual. This would be your "protocols binder" for lab-specific SOP's or protocols. It should also include generic SOPs for example the use of a BSC, emergency procedures etc, spill cleanup etc. Have all of these SOPs ready for inspection.

Q6. A 'lab rules' poster can be found here. Please print, use as an SOP and post in the lab.

Q7. If contacts are worn, goggles are mandatory for procedures which may produces spills, splashes are aerosols. Have a sign or an SOP indicating this fact in your laboratory which is endorsed by the supervisor. Have googles ready for inspection.

Q8. See Q6.

Q9. Labcoats should be available for all persons entering the laboratory including visitors. There should be a mechanism of storage of these labcoats inside the laboratory.

Q10. Signage on door should indicate requirement for closed toe and closed heel shoes to enter the laboratory.

Q11. Have goggles ready for inspection.

Q12. See Q6.

Q13. Contaminated labcoats or other clothing must be autoclaved before laundering. If no autoclave is availble for decontamination, the garment must be soaked in a bin of decontamination solution for an appropriate amount of time, rinsed, let dry, then sent for laundering. An SOP for decontamination and disposition of labcoats should be available to reflect the details specific to your laboratory. Have it ready for inspection.

Q14. See Q6.

Q15. Handwashing poster is found here.

Q16. Expectations of cleanliness and responsibility for such cleanliness should be made clearly to all workers. ("Clean up after yourself!").

Q17. If you are in a McMaster (non-hospital) hosted location, you may call the Customer Service Centre of Facilities Services at 905-525-9140 x24740 to arrange for pest control services. If you work in a hospital-hosted location, you must contact your facilities department for pest control. This number must be readily available by the phone.

Q18. Your cold units that store BSL2 agents should be lockable if kept in a room that is not lockable. The cold units must be clearly labelled as containing BSL2 agents.

Q19. -

Q20. Laboratory doors should be clearly marked with up to date contact information and PPE required for entry.

Q21. -

Q22. -

Q23. -

Q24. There should be a defined wet area and defined paperwork areas within labs that do not have separate spaces for workers to carry out non wet-lab functions.

Q25. -

Q26. -

Q27. -

Q28. You can test this by holding a kimwipe to the outside of lab door frame. Open the door a crack and if the kimwipe pulls towards the laboratory, air flow is inward. If the kimwipe blows towards you, air flow is outward.

Q29. -

Q30. The most convenient hooks to use are 3M Command Hooks, which do not damage the walls when removed and can be repositioned as needed. Hooks should be provided by the laboratory supervisor.

Q31. -

Q32. -

Q33. -

Q34. Do lab workers know where the eyewash station is? Do they know how to use it? Is the eyewash station inspected regularly? NOTE: The cardboard tag wired to the station is for facilities staff inspections. A sheet taped to the wall near the eyewash station is for lab workers to sign off on more regular (weekly) inspections. Do the lab workers know where the shower stations is? Do they know how to use it? Is it inspected regularly? Showers are typically inspected by Facility Services employees.

Q35. -

Q36. Hazards of aerosols are explained here. Reduction of aerosol generation poster is found here. Please have the poster available in your laboratory.

Q37. Do you have BSC's listed on your Biohazard Utilization Protocol (BUP)? Have you submitted your BUP?

Q38. Demonstrate where the copies of BSC certification are kept.

Q39. -

Q40. Checklist for working in a BSC is found here. Have it posted at the BSC.

Q41. See Q40.

Q42. See Q40.

Q43. Transport containers may be purchased through the biosafety office. Alternatively, containers that have a gasket and a pressure-fitted lid may be purchased from an outside source. The container must be cleanable and resistant to the disinfectants you use. Have it ready for inspection.

Q44. Decontaminants for pathogens are listed in the pathogen safety data sheets. Ensure your methods are effective. Demonstrate where your stocks of decontamination solutions are kept.

Q45. See Q44.

Q46. See Q6.

Q47. See Q6.

Q48. -

Q49. -

Q50. Sharps safety poster is found here. The "Needles and Sharps" handout is found here. A sharps container must be puncture resistant, closable and leakproof. Sharps should NOT be recapped. Have it ready for inspection.

Q51. Decontamination procedures for your contaminated waste should be outlined in your SOPs. Have it ready for inspection. Packaging before disposal of your decontaminated wastes should also be outlined in your SOPs. More information is found here. A "waste disposal" SOP to cover all wastes generated by all procedures would be ideal.

Q52. Name the company that picks up your packaged biohazardous wastes. It will be listed on the outside of the cardboard box, that is lined with a red bag, that resides in the local waste closet. Example Daniels or Stericycle. Note: Housekeeping staff my MOVE the waste to a common holding location but they do not dispose of it.

Q53. Do you use an autoclave to sterilize your waste before discard? The common autoclave that prepares sterile media and solutions is typically NOT also used to autoclave infectious waste.

Q54. If you use/own an autoclave.

Q55. See Q54.

Q56. See Q54.

Q57. Have the emergency SOPs which are relevant to your laboratory ready for inspection.

Q58. See Q57.

Q59. See Q57.

Q60. See Q57.

Q61. Do workers have all required training? Have the training records ready for inspection.

Q62. Are SOPs signed by the relevant workers?

Q63. The biosecurity plan must be signed by ALL workers. Have it ready for inspection.

Q64. Personal training record is found here. It must be maintained by the WORKER and SUPERVISOR. There is also a template for any lab specific training here. Finally there is also a template for any lab specific BIOSAFETY training here. Have it ready for inspection.

Q65. This is accomplished by BSL2 / WHMIS / Fire Safety training and regular updates.

Q66. See Q40.

Q67. -

Q68. Are undergraduate students, thesis students, volunteers and otherwise temporary workers trained in the risks expected to be present in their work area? How is this accomplished? What about use of facilities? Forms for all such scenarios may be found here.

Q69. -

Q70. -

Q71. See Q43.

The following questions refer to the CAF or other spaces in which you carry out your animal work.

Q72. -

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Q113. -

The following questions refer to large scale (over 10L) production facilities.

Q114. -

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Q130. -

The following questions refer to recombinants and genetic manipulation of pathogens.

Q131. -

Q132. Refer to LBG section 7.2

Q133. Refer to LBG section 7.2

Q134. Refer to LBG section 7.2

The following questions refer to cell culture or other in vitro work.

Q135. -

Q136. Refer to LBG section 7.3

Q137. -

Q138. -

Q139. Macaca, Rhesus macaque

Q140. -

Q141. Bovine = cow

Q142. -

Q143. A self-self experiment is one in which tissues or other samples are taken from the worker and manipulated. The worker is at risk because they have no immune defenses against their own samples even if they are modified ex vivo.