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McMaster University

Clean Culture Initiative

Cell line validation issues and working safely with cell lines is important. The documents posted here also discuss the challenges and offer potential methods of cell line validation.

Authenticating Cell Lines

NIH Notice on Authentication of Cell Lines

Open Letter on Cell Line Identification

Cell Line Cross Contamination Article

Cases of Mistaken Identity Article

Authenticating Cell Lines Article

 

Technical Resources for Cell Lines

Cell culture basics - Gibco

Cell culture and other protocols - Abcam

Cell culture protocols - Invitrogen

Cell culture techinques - Sigma

 

Test Your Cell Lines

VirOligo - primer database, test your cell lines for pathogens

h-IMPACT - have them test your cell lines for human viral pathogens

[Article] Development of RT-PCR Array for Simultaneous Detection of Eight Human Blood-Borne Viral Pathogens

 

 

 

Working with Cell Lines

Alternate Method to Iso enzyme Profile

ECVAM Guidance on Good Culture Practices 
2nd Task Force 2005

Herpes B in Fluids

Standard Safety Practices Sorting Unfixed Cell Lines

Reference Web Sites for Cell Lines

ATCC Home Page

Geoff Werstuck Lab

Cell Biology Lab Manual - Gustavus Adolphus College

Journal of Visualized Experiments - JOVE

 

Microbiology Links

Online Textbook of Bacteriology - searchable and browseable

Medical Microbiology, 4th Edition - online textbook from NCBI

ASM Microbe Library - online library by the American Society for Microbiology

Micro Techniques - nice pictures demonstrating techniques

 

 

Updates on the changing HPTA regulations, the new Canadian Biosafety Standards and Guidelines and more click here